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Effect of read depth on variant calling in gene-rich regions in Arabidopsis. (A) Effect of mean read depth (RD) on template coverage (C15, percentage of template sequence read with RD ≥ 15×). (B, C) Effect of mean RD on the (B) precision and (C) sensitivity of the analysis. Precision is calculated as TP/(TP + FP), and sensitivity as TP/(TP + FN). TP: True positive SNPs. FP: False positive SNPs. FN: False negative SNPs.
