Figure 5.
Premature ribosomes form distinct initiation complexes. (A) A stacked bar plot representing the distribution of initiator (tRNAifmet) and elongator (tRNAe) tRNAs in the 30S and 70S derived fractions as observed in the TCP-seq data. Read counts were converted to Reads per million (RPM). (B) An outline of the methodology used to capture translation initiation and elongation complexes on an mRNA harbouring AGG start codon. (C and D) Distribution of the AGG-GFP mRNA derived FPs from the 30S (C) and 70S fractions (D) of Wt, ΔrbfA and ΔksgA. The FP counts are shown along the entire length of the gene encoding GFP.