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. 2019 Oct 5;47(21):11368–11386. doi: 10.1093/nar/gkz825

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Elevated levels of initiation factors avert premature ribosomes from engaging in translation. (A–C) IRI measurements for Wt, ΔrbfA and ΔksgA using gfp that harbours AUG or AGG start codon in presence of elevated cellular concentrations of IF1 (A), IF2 (B) and IF3 (C) are presented. The statistical significance was tested using a paired t-test analysis with a 95% confidence interval. (D–F) Representative polysome profiles for Wt, ΔrbfA or ΔksgA cells carrying an empty p15A vector (only Wt, ΔrbfA or ΔksgA) or p15A vector carrying genes encoding IF1, IF2 or IF3 are shown. The inset shows the profile for the respective null mutants carrying p15A vector carrying a gene encoding RbfA or KsgA. The profiles were drawn from mid-log phase cells overexpressing the respective initiation factors.