Skip to main content
. 2019 Oct 31;47(21):11197–11208. doi: 10.1093/nar/gkz961

Figure 1.

Figure 1.

Cell Cycle genes are deregulated after DNA damage in cells lacking RB. (A) Immunoblot of HFFs with CRISPR-Cas9 mediated knockout of p130 and/or RB in contact arrest and serum starvation conditions. (B) Immunoblot of Control, sgP130, sgRB1, or sgRB1+sgP130 cells split into doxorubicin and harvested after 24 h. (C) Same as (B) but cell cycle phase was determined by BrdU incorporation and PI stain (n = 3). (D) Immunoblot of lysates prepared from p130, LIN9 and IgG immunoprecipitations using lysates from HFFs split into doxorubicin and harvested after 24 h. (E) RNA-seq of Control, sgP130, sgRB1, or sgRB1+sgP130 cells split into doxorubicin and harvested 24 h later (n = 2). Heatmap comparing top 250 differentially expressed genes amongst doxorubicin treated samples for each cell line with G1/S, G2/M or p53 target gene categorization indicated. (F) Volcano plot of differentially expressed genes from (E) with cut off of Log2 fold change of 1.5 and adjusted P value of 0.0001. See also Supplemental Figure S1.