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. 2019 Oct 31;47(21):11197–11208. doi: 10.1093/nar/gkz961

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — P107 repression of G2/M genes prevents mitotic entry. (A–C) RT-qPCR of HFFs with indicated genotype transfected with siRNA and harvested after 48 h of contact arrest and serum starvation (n = 3). (D) sgRB1+sgP130 cells were transfected with siRNA, contact arrested for 48 h, and then were split into nocodazole and harvested at 30 h and analyzed by flow cytometry using antibody against phospho-H3 and propidium iodide. (E) Quantification of pH3+ cells in D. Student's t-test was used to measure significance between indicated samples (n = 3). P values are as indicated as * for <0.05, ** for <0.01 and *** for <0.001. NS indicates P value >0.05.