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. 2019 Sep 13;68(12):2259–2271. doi: 10.2337/db19-0117

Figure 1.

Figure 1

Murine NEUROD1 is required for the late fetal expansion of pancreatic INSULIN+ and GLUCAGON+ cells. A: IF expression analysis of indicated markers and TUNEL staining performed on sections of E15, E17, and E18 pancreata. Scale bar = 50 μm. B: Quantification of the percent of insulin+ cells that are TUNEL positive. C: Quantification of INSULIN+ cells expressing Ki67. The total number of Ins+ cells counted per Neurod1+/+ sample was E18 = 554 ± 162, E17 = 347 ± 184, and E15 = 308 ± 161 and per Neurod1−/− was E18 = 548 ± 102, E17 = 344 ± 100, and E15 = 293 ± 36. D: Quantification of GLUCAGON+ cells expressing Ki67. The total number of GCG+ cells counted per Neurod1+/+ sample was E18 = 207 ± 89, E17 = 94 ± 49, and E15 = 220 ± 121 and per Neurod1−/− was E18 = 112 ± 45, E17 = 108 ± 42, and E15 = 111 ± 57. All error bars indicate SD. Student t test P value compares values of controls to those of indicated genotype. *P < 0.01; **P < 0.005.