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. 2019 Sep 13;68(12):2259–2271. doi: 10.2337/db19-0117

Figure 4.

Figure 4

Impaired differentiation of NEUROD1mut/mut HESC-derived pancreatic progenitors into insulin-expressing cells. A: IF of pancreatic progenitor markers PDX1 and NKX6.1 at day 12 of differentiation. Scale bar = 100 μm. B: Quantification of PDX1+, NKX6-1+ cells at day 12 of differentiation. C: Flow cytometry expression analysis and quantification of lineage markers at day 12 of HESC-β differentiation. D. Flow cytometry expression analysis of lineage markers at day 18 of HESC-β differentiation. Scale bar = 50 μm. n = 4 for each genotype. Error bars indicate SD. Student t test P value compares values of controls to NEUROD1mut/mut. scBeta, stem cell–derived β-cells. *P < 0.01.