Figure 6.

NEUROD1^mut/mut and control HESC-β cells have similar low rates of proliferation and apoptosis. A: Flow cytometry histograms of EDU incorporation for C-peptide⁺ cells at different stages of HESC-β cell differentiation as well as for OCT4⁺ cells in ESCs. B: Quantification of proliferating EDU⁺ C-peptide⁺ cells determined by flow cytometry analysis. C: TUNEL and IF staining for Ki67 and insulin on sections of day 27 (D27) HESC–derived endocrine clusters. Yellow arrow points to Ki67⁺ insulin⁺ cells. White arrow points to TUNEL⁺ INSULIN⁺ cell D: Quantification of apoptotic TUNEL⁺ INSULIN⁺ cells. E: Quantification of INSULIN⁺ cells expressing proliferation marker Ki67.