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. 2019 Nov;19(4):355–367. doi: 10.17305/bjbms.2019.4227

FIGURE 1.

FIGURE 1

Gefitinib-resistant persisters (GRPs) of PC9 and HCC827 cells express high levels of F-box/WD repeat-containing protein 7 (FBXW7) and CD133 but a low level of c-MYC. (A) The mRNA expression of FBXW7 and PROM1 (CD133) in PC9 and HCC827 cells was analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) 9 days after exposure to gefitinib. Expression levels of FBXW7 and CD133 in parental cells and in GRPs of PC9 and HCC827 were compared. Data were normalized to actin expression. All values represent the average of triplicate experiments. Average values for each parental cell line were set to 1. *p < 0.05, **p < 0.01. (B) Immunofluorescence of FBXW7 and c-MYC in PC9 and HCC827 cells. Cells were cultured with 1 µM gefitinib on slides of Labo-Tek chamber for 72 h. Nuclei were stained with DAPI. (C) The ratio of FBXW7-positive or c-MYC-positive cells in PC-9 and HCC827 cells. Parental cells and GRPs from each cell line were compared. Average values were calculated from five fields in each sample. **p < 0.01; scale bar indicates 50 µm.