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. Both F-box/WD repeat-containing protein 7 (FBXW7) and CD133 proteins were highly expressed in gefitinib-resistant persisters (GRPs) of PC9 fluorescent ubiquitination-based cell cycle indicator (FUCCI) and HCC827 FUCCI. The fluorescence image of GRPs showed red color, indicating that GRPs existed in G0/G1 phase. Cells were seeded in 35 mm glass bottom dishes, followed by treatment with or without 1 µM gefitinib for 72 h. Scale bar indicates 20 µm.
