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Cold-induced dispersal of cytoophidia is reversible and fast. It takes 3 min and 45 s to prepare the sample before confocal capture on the video. This time period should be added prior to the starting time point. The cells are transferred from 0 °C to 32 °C to perform the recovery experiment. Within 5 min cytoophidia (CTPS, green) start to emerge, indicating a quick response to temperature changes in S. pombe.
