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. 2019 Nov 14;10:1314. doi: 10.3389/fphar.2019.01314

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Copyright © 2019 Figueroa, Lozano, Lobos, Hennrikus, Gonzalez and Amador

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Unilateral ureteral obstruction (UUO) induces upregulation of renin in left renal cortex. (A) mRNA levels for: angiotensinogen (AGT), renin, angiotensin-converting enzyme (ACE)1/2, (pro)renin receptor (PRR), and angiotensin-II receptor type 1 (AT1R) in the renal cortex. 18S mRNA was used as the housekeeping gene in qRT-PCR studies. Western blot and immunodetection of (B) renin and (C) ACE1 in the renal cortex. α-tubulin was used as internal loading control. Student’s t test was performed. **P < 0.01 or ***P < 0.001 vs. Sham group (n = 5).