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UUO induces downregulation of (pro)renin receptor (PRR) in left renal medulla. (A) mRNA levels for: AGT, renin, ACE1/2, PRR, and AT1R in renal medulla. 18S mRNA was used as the housekeeping gene in qRT-PCR studies. Western blot and immunodetection of (B) AGT and (C) PRR in renal medulla. α-tubulin was used as internal loading control. Student’s t test was performed. *P < 0.05 or **P < 0.01 vs. Sham group (n = 5).
