Figure 4.

The blockage of miR-21 abolishes the inhibitory effects of LGS on STAT3 mRNA (A) and IL-6 mRNA (B). The cells were transfected with miR-21 mimic or inhibitor for 24 h. After transfection, macrophages were cultured with LGS (200 μg/ml) and LPS (1 μg/ml) for another 24 h. The mRNA expressions of STAT3 and IL-6 were measured by qPCR. Data are represented as the mean ± SEM of three independent experiments. ^## P < 0.01 and ^### P < 0.001 versus control, ^* P < 0.05, ^** P < 0.01 versus LPS treatment, ⁺ P < 0.05 versus LGS treatment by one-way ANOVA with Tukey’s test.