Figure 7.

LGS inhibits the STAT3 signaling pathway by up-regulating miR-21 in ALI mice. Forty-eight mice were randomly divided into six groups each experiment. (A co-worker blinded to the experimental protocol randomized animals, n = 8/group.) After sacrifice, the lung tissues in ALI mice were harvested and the total proteins and RNA were extracted, respectively. (A) LGS inhibits the STAT3 signaling pathway in ALI mice. The protein expressions of STAT3 and P-STAT3 (Tyr705) were detected by Western blotting. (B) LGS enhances the expression of miR-21 in ALI mice. The expression of miR-21 in lung tissues was measured by qPCR. Data are represented as the mean ± SEM of three independent experiments. ^### P 0.001 versus control, *P < 0.05, **P < 0.01 and versus LPS treatment by one-way ANOVA with Tukey’s test.