Figure 3.

Migration behavior of SC in RGD and YIGSR concentration gradients. Time-lapse microscopy was used to track the position of individual cells. Images were taken every 10 min for a total of 24 h per experiment. (A, B, C, D, E) Centroid tracks from 30 representative cells in a typical experiment for YIGSR gradient, uniform YIGSR, RGD gradient, uniform RGD, and uniform laminin, respectively, with initial position of each track superimposed at 0,0 for clarity. (F) Polar plot showing the end point of each cell’s trajectory relative to its origin. Cells moving upward move toward the positive gradient of concentration of the respective peptide. (G) Mean square displacement curves for over 24 h period. (H) Overall velocity and bias velocity to the direction of the gradient μm/min (ANOVA with Tukey’s). Capital letter were used to correlate velocity in the x direction; lowercase letter were used to correlate velocity in the y direction; and Greek letters were used to correlate overall speed. Means that not share a letter were significant different. YIGSR concentration gradients had a positive influence on directing Schwann cell migration.