Figure 7.

Yeast assay showing the effect of hGPR132a residue mutagenesis on the agonist potency of ligands: (A) SB‐583831, (B) CP‐55,940, (C) NPGly, (D) NLGly. hGPR132a sequences are represented as follows: K183A (purple), Y199A (cyan), Y200A (blue), R203A (green), and wild‐type (black). Response data for each mutant were normalized to effects of the corresponding ligand at wild‐type hGPR132a. SB‐583831 did not activate mutant R203A but showed an inverse agonist concentration‐response, inhibiting the elevated basal signal (elevated basal is due to receptor constitutive activity). Data are presented as mean ± SEM of n = 3 independent experiments