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. 2019 Nov 21;13:90. doi: 10.1186/s13036-019-0217-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — CRISPR/Cas9 induces targeted DNA cleavage in chicken cells. a Co-transfection of U6-gRNA-pHEf1A-Cas9-mKate and EYFP-Truncated plasmids in chicken DF-1 (48 h); b Flow cytometry analysis of EYFP percentage in co-transfected DF-1 cells in (a); c Experimental scheme for targeted DAZL gene knock out in DF-1 cells; d T7 endonuclease I assay of the DAZL gene mutation in chicken DF-1 cells; e Sequencing analysis of targeted mutation in the DAZL gene