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. 2019 Nov 21;13:90. doi: 10.1186/s13036-019-0217-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — HMEJ mediates robust gene integration in chicken PGCs. a and b Plasmid design for gene integration at the DAZL and Pou5f3 loci; c Efficiency of HITI, HDR and HMEJ gene integration at the DAZL and Pou5f3 genes in PGCs; d PGCs bearing stable integration at DAZL and Pou5f3; e PCR of the site-specific gene integration in DAZL-2A-mCherry and Pou5f3-2A-mCherry cell lines; f Sequencing analysis of site-specific gene integration; G and H. The gonad migration of Pou5f3-2A-mCherry (g) and DAZL-2A-mCherry (h) PGCs were observed in 7 days embryos