Fig. 6.

LncRNA-HGBC increases the expression of oncoprotein SET via competitively binding to miR-502-3p. a Dual luciferase assay was performed in NOZ cells cotransfected with miR-502-3p mimics or lncRNA-HGBC expressing plasmid and luciferase reporters containing 3’UTR of SET. b SET mRNA (top) and protein (bottom) levels in stably NOZ and EH-GB1 cell clones transduced with indicated DNA or RNAs. c CCK-8 assays in stable lncRNA-HGBC-depleted NOZ or stable lncRNA-HGBC-overexpressing EH-GB1 cells transfected with indicated miR-502-3p inhibitors or mimics, respectively. d Transwell migration and invasion assays were tested in NOZ cells transfected with shlncRNA-HGBC and miR-502-3p inhibitor. Scale bars, 200 μm. e Transwell migration and invasion assays in lncRNA-HGBC-overexpressing EH-GB1 cells were performed in co-transfection of miR-502-3p mimics. Scale bars, 200 μm. f, g SET mRNA (f) and protein (g) levels after transfection of the indicated plasmids into GBC-SD and EH-GB1 cells. *P < 0.05, **P < 0.01, ***P < 0.001