Fig. 8.

Gene expression of the osteoblastic markers. The target genes were normalized to the reference gene GAPDH using the der ΔCt method with ΔCt = Ct test gene − Ct reference gene (GAPDH) and ΔΔCt = ΔCt sample − ΔCt calibrator (unstimulated cells). The relative quantification (RQ) (=fold change compared to the calibrator) was calculated as 2^-ΔΔCt. The figure shows the single values of n = 10 patients as symbols with the median as line. BM bone marrow, CB cancellous bone, VF vacuum filter, SF cell saver filtrate reservoir. a RQ of RUNX. b RQ of ALP. c RQ of BMP-2