Figure 2. The effect of MIF knockdown by specific siRNA on mediators of trophoblast invasion in HTR-8/SVneo cells. Integrin subunit α1 was significantly reduced, while subunits α5 and β1 were not changed as assessed by flow cytomerty (a). Gelatine zymography showed a significant decrease in both MMP2 (b) and MMP9 (c) levels. Representative zymogram is shown in d. MFI - mean fluorescent intensity. Data are presented as mean +SEM, * p<0.05, ** p<0.01, *** p<0.001, n =3-6.
