Figure 2.

MCT4 destroys epithelial barrier function and inhibits ZO‐1 expression. A, Transepithelial electrical resistance was detected over time in CaCO₂ cells treated as indicated. B, Cy7‐dextran paracellular intestinal epithelial permeability was performed at 28 days post incubation. C, Immunofluorescence staining of tight junction protein ZO‐1 in CaCO₂ cells was grown to confluence in indicated group. D, ZO‐1 mRNA level was analysed in real‐time PCR analysis in CaCO₂ cells, scale bar: 50 μm. E‐F, Western blotting was employed to detect ZO‐1 expression in stable HT‐29 and CaCO₂ cells with MCT4 overexpression