Figure 6.

MCT4 modulates the interaction of CBP and NF‐κB or CREB. A, Co‐localization of endogenous CBP and NF‐κB by immunofluorescence performed in HT‐29 cells treated as indicated, respectively, scale bar: 200 μm. B, CaCO₂ cells were grown to 80% confluence and replaced with medium without serum for 18 h, and then stimulated with DMSO or CHC for 1 h; whole cell lysates were immunoprecipitated (IP) with antibodies against endogenous CBP; and NF‐κB and CREB were detected by immunoblotting. C, Indicated stable CaCO₂ cells were grown to 80% confluence, whole cell lysates were immunoprecipitated (IP) with antibodies against endogenous CBP, and co‐precipitates with NF‐κB/CREB were detected by immunoblotting