Table 1.
primer for indicated genes in real‐time PCR assay
| Gene | Forward | Reverse |
|---|---|---|
| ZO‐1 | TGTGAGTCCTTCAGCTGTGGAA | GGAACTCAACACACCATTG |
| IL‐1β | GTAGCCCACGTCGTAGCAAA | CCCTTCTCCAGCTGGGAGAC |
| IL‐6 | GGAACTCAACACACCATTG | GTCAGGTCGGACTCCCGAGAA |
| IL‐8 | GCAGCTCTGTGTGAAGGTGCAGTTT | CTCAGCCCTCTTCAAAAACTTCTCC |
| TNF‐α | CAGAGGGAAGAGTTCCCCAG | CCTTGGTCTGGTAGGAGACG |
| COX‐2 | TGCATTCTTTGCCCAGCACT | AAAGGCGCAGTTTACGCTGT |
| IL‐10 | ACAGGGAAGAAATCGATGACA | TGGGGGAGAACCTGAAGAC |
| MCT4 | GAGTTTGGGATCGGCTACAG | CGGTTCACGCACACACTG |
| TJP2 | GGGAAGGTCGCTGCTATTGT | CTCTCGCTGTAGCCACTCC |
| JAM | TGTTTCAGTTCTGTGTCATGGT | TGCAGACAAGGTGTTTTCCAG |
| Occludin | ACAAGCGGTTTTATCCAGAGTC | GTCATCCACAGGCGAAGTTAAT |
| E‐cadherin | TGCCCAGAAAATGAAAAAGG | GTGTATGTGGCAATGCGTTC |
| UBC | ATTTGGGTCGCGGTTCTTG | TGCCTTGACATTCTCGATGGT |