Table 2.
GO terms for biological process enriched in the set of genes differentially expressed across monocyte subsets
| Preinfection | 26 dpi | Necropsy | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| GO term | Subset | # | % | P | # | % | P | # | % | P |
| Defense response | C | 38 | 7.6 | *** | 36 | 5.8 | ** | 41 | 7.7 | **** |
| GO:0006952 | I | 40 | 8.8 | **** | 40 | 6.4 | *** | 36 | 8.1 | **** |
| N | 25 | 9.5 | **** | 22 | 13.4 | **** | 9 | 8.0 | * | |
| Inflammatory response | C | 24 | 4.8 | *** | 27 | 4.3 | *** | 28 | 5.2 | **** |
| GO:0006954 | I | 24 | 5.3 | **** | 29 | 4.6 | **** | 27 | 6.1 | **** |
| N | 13 | 4.9 | ** | 13 | 7.9 | *** | 7 | 6.3 | * | |
| Regulation of T cell activation | C | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| GO:0050863 | I | 9 | 2.0 | * | 10 | 1.6 | * | 8 | 1.8 | * |
| N | 13 | 4.9 | **** | 9 | 5.5 | **** | 7 | 6.3 | **** | |
| Cell activation | C | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| GO:0001775 | I | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| N | 17 | 6.4 | **** | 8 | 4.9 | * | 11 | 9.8 | **** | |
| Positive regulation of cell proliferation | C | 27 | 5.4 | *** | 26 | 4.2 | ** | 26 | 4.9 | ** |
| GO:0008284 | I | 21 | 4.6 | * | 28 | 4.5 | ** | 21 | 4.7 | ** |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Negative regulation of cell proliferation | C | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| GO:0008285 | I | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| N | 15 | 5.7 | ** | 12 | 7.3 | ** | 10 | 8.9 | *** | |
| Positive regulation of apoptosis | C | 24 | 4.8 | ** | NS | NS | NS | NS | NS | NS |
| GO:0043065 | I | NS | NS | NS | 26 | 4.1 | * | NS | NS | NS |
| N | 15 | 5.7 | ** | NS | NS | NS | 8 | 7.1 | * | |
| Negative regulation of apoptosis | C | 25 | 5.0 | *** | 22 | 3.5 | * | 22 | 4.1 | ** |
| GO:0043066 | I | 20 | 4.4 | ** | 21 | 3.3 | * | 18 | 4.0 | * |
| N | 12 | 4.5 | * | 9 | 5.5 | * | 7 | 6.3 | * | |
| Positive regulation of cell differentiation | C | 17 | 3.4 | ** | 16 | 2.6 | * | 17 | 3.2 | ** |
| GO:0045597 | I | 15 | 3.3 | ** | 16 | 2.5 | * | 13 | 2.9 | * |
| N | 10 | 3.8 | * | NS | NS | NS | 5 | 4.5 | * | |
| Wound healing | C | 21 | 4.2 | **** | 15 | 2.4 | ** | 17 | 3.2 | *** |
| GO:0042060 | I | 18 | 4.0 | **** | 13 | 2.1 | * | 11 | 2.5 | * |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Angiogenesis | C | 11 | 2.2 | * | 13 | 2.1 | ** | 12 | 2.2 | ** |
| GO:0001525 | I | NS | NS | NS | 12 | 1.9 | * | 11 | 2.5 | ** |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Endocytosis | C | 16 | 3.2 | ** | 20 | 3.2 | *** | 18 | 3.4 | *** |
| GO:0006897 | I | 16 | 3.5 | ** | 21 | 3.3 | *** | 16 | 3.6 | *** |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Membrane organization | C | 29 | 5.8 | **** | 31 | 5.0 | **** | 27 | 5.0 | *** |
| GO:0016044 | I | 27 | 6.0 | **** | 32 | 5.1 | **** | 24 | 5.4 | *** |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Vesicle‐mediated transport | C | 35 | 7.0 | *** | 39 | 6.3 | *** | 33 | 6.2 | ** |
| GO:0016192 | I | 30 | 6.6 | ** | 40 | 6.4 | *** | 29 | 6.5 | ** |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Phagocytosis | C | NS | NS | NS | 7 | 1.1 | ** | 6 | 1.1 | * |
| GO:0006909 | I | 6 | 1.3 | ** | 7 | 1.1 | ** | 6 | 1.3 | ** |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Regulation of protein kinase cascade | C | 23 | 4.6 | **** | 18 | 2.9 | ** | 19 | 3.6 | ** |
| GO:0010627 | I | 18 | 4.0 | *** | 18 | 2.9 | * | 13 | 2.9 | * |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Regulation of MAP kinase activity | C | 12 | 2.4 | ** | 12 | 1.9 | ** | NS | NS | NS |
| GO:0043405 | I | 9 | 2.0 | * | 13 | 2.1 | ** | 16 | 3.6 | * |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Regulation of I‐kappaB kinase/ NFkB cascade | C | 10 | 2.0 | ** | NS | NS | NS | 10 | 1.9 | ** |
| I | 9 | 2.0 | ** | NS | NS | NS | NS | NS | NS | |
| GO:0043122 | N | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| Positive regulation of cytokine production | C | 12 | 2.4 | **** | 10 | 1.6 | ** | NS | NS | NS |
| GO:0001819 | I | 12 | 2.6 | **** | 11 | 1.7 | ** | NS | NS | NS |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Cell adhesion | C | 31 | 6.2 | * | NS | NS | NS | NS | NS | NS |
| GO:0007155 | I | 29 | 6.4 | * | NS | NS | NS | NS | NS | NS |
| N | 20 | 7.6 | * | NS | NS | NS | 10 | 8.9 | * | |
| Chemotaxis | C | 12 | 2.4 | ** | 12 | 1.9 | * | 12 | 2.2 | * |
| GO:0006935 | I | 12 | 2.6 | ** | 13 | 2.1 | * | NS | NS | NS |
| N | NS | NS | NS | 7 | 4.3 | ** | NS | NS | NS | |
| Cell migration | C | 19 | 3.8 | ** | 18 | 2.9 | * | 18 | 3.4 | ** |
| GO:0016477 | I | 14 | 3.1 | * | NS | NS | NS | 15 | 3.4 | * |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
| Actin cytoskeleton organization | C | 13 | 2.6 | * | 15 | 2.4 | * | 15 | 2.8 | * |
| GO:0030036 | I | 13 | 2.9 | * | 12 | 1.9 | * | 14 | 3.1 | ** |
| N | 10 | 3.8 | ** | NS | NS | NS | NS | NS | NS | |
| Posttranscriptional regulation of gene expression | C | NS | NS | NS | 18 | 2.9 | ** | 15 | 2.8 | ** |
| GO:0010608 | I | NS | NS | NS | 17 | 2.7 | ** | 12 | 2.7 | * |
| N | NS | NS | NS | NS | NS | NS | NS | NS | NS | |
C, classical; I, intermediate; N, nonclassical; #, number of queried genes associated with the GO term; %, (# GO associated genes/total genes queried) × 100; NS, not significant; P‐value for enrichment of the GO term: * P ≤ 0.05, ** P < 0.01 *** P < 0.001, P < 0.0001.