Figure 5.

Effects of miR‐29c‐3p on gastric cancer cells proliferation, cell cycle and apoptosis in vitro. A, MiR‐29c‐3p expression was detected in SGC‐7901 and BGC‐823 cells by qRT‐PCR after transfection of miR‐29c‐3p mimic, miR‐29c‐3p inhibitor or control miRNA. B, After transfection of the miR‐29c‐3p mimetic, miR‐29c‐3p inhibitor or control miRNA, lncRNA MYOSLID expression was detected in SGC‐7901 and BGC‐823 cells by qRT‐PCR. C, After transfection of miR‐29c‐3p mimic, miR‐29c‐3p inhibitor or control miRNA, the proliferation of SGC‐7901 and BGC‐823 cells was detected by CCK‐8. D, Flow cytometry apoptosis assay was used to analyse apoptosis in BGC‐823 and SGC‐7901 cells transfected with miR‐29c‐3p mimics. E, Flow cytometry assays were performed to analyse cell cycle progression when miK‐29c‐3p was used to mimic transfected SGC‐7901 and BGC‐823 cells. F, The growth curve of SGC7901 cells was co‐transfected with LV‐MYOSLID, miR‐29c‐3p mimics or scrambled siRNA by CCK8. (G). The growth curve of BGC‐823 cells was co‐transfected with si‐MYOSLID 2#, miR‐29c‐3p inhibitor or scrambled siRNA by CCK8. H, The colony‐forming ability of BGC‐823 cells was co‐transfected with si‐MYOSLID 2#, miR‐29c‐3p inhibitor or scrambled siRNA by colony formation assay. I, The colony‐forming ability of SGC‐7901 cells after co‐transfection with LV‐MYOSLID, miR‐29c‐3p mimics or scrambled siRNA was determined by colony formation assay. Values represent the mean ± SEM of three independent experiments. *P < .05, **P < .01, **P < .001