Figure 5.

CB1 upregulated the osteo/dentinogenic differentiation potential of PDLSCs under TNF‐α and INF‐γ stimulation. A‐D, 10 ng/mL TNF‐α was used to treat PDLSCs. A, Real‐time RT‐PCR results showed the expression of CB1 at 1, 2, 4 and 8 h after 10 ng/mL TNF‐α treatment in PDLSCs. B, ALP activity assay. C, Alizarin Red staining. D, Calcium quantitative analysis. E‐H, 100 ng/mL INF‐γ was used to treat PDLSCs. E, Real‐time RT‐PCR results showed the expression of CB1 at 1, 2, 4 and 8 h after 100 ng/mL INF‐γ treatment in PDLSCs. F, ALP activity assay. G, Alizarin Red staining. H, Calcium quantitative analysis. GAPDH was used as an internal control. One‐way ANOVA was performed to determine statistical significance. Error bars represent the SD (n = 3). *P ≤ .05; **P ≤ .01