Figure 6.

USF1 activated SOX2 expression at the transcriptional level. A, With the aid of UCSC database, it was found that USF1 might bind with the promoter region of SOX2. B, The binding capability of USF1 to SOX2 promoter was analysed by ChIP assay. C, RT‐qPCR was adopted to evaluate the transfection efficiency. D, The luciferase activity of SOX2 promoter was determined by luciferase reporter assays when USF1 was silenced or overexpressed. E, The regulation of USF1 on SOX2 expression was estimated by RT‐qPCR. ^* P < .05, ^** P < .01, ^*** P < .001