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. 2019 Nov 14;10:1032. doi: 10.3389/fgene.2019.01032

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Copyright © 2019 Freem, Summers, Gheyas, Psifidi, Boulton, MacCallum, Harne, O’Dell, Bush and Hume

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Variation in blood leukocyte populations in F2 progeny of F1 sibling mating. Blood from the entire cohort of hatchling birds was analyzed for total CSF1R-mApple reporter gene expression (which measures the myeloid compartment including heterophils) or for blood monocytes (KUL01/mApple+). For logistical reasons these analyses were performed on Day 1 or Day 2 of hatch. Bars show the mean +/- SD. The Figure demonstrates that the variance between birds was small, there was no effect of the sex or day of sampling. (A) Blood leukocytes in males and females. (B) Blood leukocytes on Day 1 and Day 2 after hatch.