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. 2019 Nov 14;10:1032. doi: 10.3389/fgene.2019.01032

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Copyright © 2019 Freem, Summers, Gheyas, Psifidi, Boulton, MacCallum, Harne, O’Dell, Bush and Hume

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Network analysis of the response of BMDM generated from adult broiler and layer chickens to LPS. RNAseq gene expression data from BMDM cultured with or without LPS for 24h was analyzed using the network visualization tool Graphia. The panels show the sample-to-sample matrix with a Pearson correlation threshold of 0.97. In the three panels birds are identified based upon breed, sex or treatment as indicated. Note that the samples segregate solely based upon LPS stimulation. The sets of LPS-regulated transcripts that contribute to this separation are summarized in Table S3 .