Table 1.

DNA sequence variants in the vicinity of candidate genes of interest detected in 27 founder broiler and layer birds.

Gene	Coordinates in Gal5	No. of Variants	No. of Indels	No. of variants with quality >30	No. HIGH MODERATE effect variants	No. of variants overlapped with constrained elements
CSF1R	Chr 13: 13275507 to 13292566	457	40	453	2	38
CSF1	Chr 26: 1276201 to 1282875	99	21	85	4	0
IL10	Chr 26: 2562275 to 2564509	39	3	38	3	5
IL34	Chr 11: 1776702 to 1781094	98	14	95	1	4

High impact variants include stop gain/loss and splice donor and acceptors. Moderate impact variants includes nonsynonymous SNVs. Constrained elements are evolutionary conserved regions of the chicken genome detected by comparative analysis of 48 bird genomes using GERP++ package (https://pag.confex.com/pag/xxiv/webprogram/Paper21473.html)