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. 2019 Nov 18;7:e8058. doi: 10.7717/peerj.8058

Figure 5. Examples of Fluorescent in situ hybridisation (FISH) images of dragonfly gut sections using bacteria-specific probes.

Figure 5

Host cell nuclei are stained purple with DAPI, eubacteria are green, and Wolbachia is pink. Arrows highlight bacteria in each section. (A) Representative brightfield image of P. flavescens midgut section showing columnar cellular folds covering the gut lumen, and food particles in the lumen. (B–D) Foregut sections of P. flavescens, O. sabina and O. pruinosum. Note the lack of eubacterial or Wolbachia signal. (E–G) Midgut and (H–J) hindgut sections of each species, stained with a eubacterial probe. Note the strong eubacterial signal near the columnar folds of P. flavescens. (K–M) Midgut and (N–P) hindgut sections of each species, stained with a Wolbachia- specific probe. Note the lack of signal in P. flavescens, a weak signal in O. pruinosum, and a large globular structure with Wolbachia in O. sabina.