Table 2.
Sequences of real-time PCR primers.
| Gene name | Sequence | GenBank no. |
|---|---|---|
| GOT | F-5′-CGTATAGGTGCCGAGTTCCT-3′ | NM_205321.1 |
| R-5′-CGTATAGGTGCCGAGTTCCT-3′ | ||
| GPT | F-5′-GGGAAATGTCTTGCGCCTAG-3′ | XM_015292423.1 |
| R-5′-GCTCTGGACAAACTGAAGCC-3′ | ||
| TDH | F-5′-AAACCTGTGACCAACGATGC-3′ | XM_420039.5 |
| R-5′-AACAGCCGCAACACTCAAAT-3′ | ||
| MUC2 | F-5′-CATTCAACGAGGAGAGCTGC-3′ | NM_001318434.1 |
| R-5′-TTCCTTGCAGCAGGAACAAC-3′ | ||
| β-gal 9 | F-5′-ACCACGCAGTTAAGAGCCCT-3′ | NM_001001611.2 |
| R-5′-CACCAGCAAATGTCAGCAATG-3′ | ||
| ZO-1 | F-5′-CCAAAGACAGCAGGAGGAGA-3′ | XM_015278981.1 |
| R-5′-TGGCTAGTTTCTCTCGTGCA-3′ | ||
| pTOR | F-5′-GGAATGAACCGTGATGACCG-3′ | NC_006108.3 |
| R-5′-AGCATTTGACTGAGAGGGCT-3′ | ||
| PepT1 | F-5′-TAGACTGGGCAAGCGAGAAG-3′ | KF366603 |
| R-5′-AGCAGCAGCAACGAAAGC-3′ | ||
| B0AT1 | F-5′-TCTGCCTGGGTTTGTCATCT-3′ | XM_419056.4 |
| R-5′-AGCCAGTAATTGCCAGACCT-3′ | ||
| ANPEP | F-5′-TGGTTGAATGAGGGCTTTGC-3′ | NM_204861.1 |
| R-5′-TGCTGGGGTGTTGATCTCAT-3′ | ||
| β-actin | F-5′-GAGAAATTGTGCGTGACATCA-3′ | NM_205518 |
| R-5′-CCTGAACCTCTCATTGCCA-3′ |
GOT: glutamic oxaloacetic transaminase; GPT: glutamic-pyruvic transaminase; TDH: threonine dehydrogenase; MUC2: mucin 2; β-gal 9: β-defensin-9; ZO-1: zonula occludens protein 1; pTOR: poultry target of rapamycin; PepT1: peptide transporter 1; B0AT1: neutral amino acid transporter; ANPEP: aminopeptidase.