Table 9.
Effects of dietary threonine levels on gene expression levels in chick embryos from breeder hens of yellow-feathered chickens.
Thr supplementation (bold) and (total dietary content, %) | P-value2 | ||||||
---|---|---|---|---|---|---|---|
Variable | 0.00 | 0.24 | 0.48 | Thr | L | Q | |
(0.38) | (0.62) | (0.86) | SEM1 | ||||
In liver | |||||||
pTOR | 1.46b | 1.82a | 1.70 a | 0.05 | 0.001 | 0.003 | 0.001 |
GOT | 0.69 | 0.94 | 0.75 | 0.05 | 0.08 | ||
GPT | 0.30 | 0.36 | 0.39 | 0.03 | 0.63 | ||
TDH | 1.74 | 1.70 | 1.63 | 0.04 | 0.85 | ||
In thigh muscle | |||||||
pTOR | 1.25 | 1.21 | 1.30 | 0.02 | 0.21 | ||
TDH | 0.59 b | 0.68a | 0.70a | 0.02 | 0.03 | 0.02 | |
In breast muscle | |||||||
pTOR | 0.18b | 0.20b | 0.30a | 0.02 | 0.04 | 0.02 | |
TDH | 0.60 | 0.65 | 0.61 | 0.01 | 0.44 | ||
In ileum | |||||||
ANPEP | 0.63b | 0.69a,b | 0.75a | 0.02 | 0.05 | 0.02 | |
B0AT1 | 0.18 | 0.14 | 0.21 | 0.02 | 0.27 | ||
MUC2 | 0.67 | 0.67 | 0.65 | 0.03 | 0.94 | ||
PepT1 | 0.59 | 0.57 | 0.59 | 0.03 | 0.96 | ||
In duodenum | |||||||
ANPEP | 0.90b | 0.95b | 1.10a | 0.03 | 0.002 | 0.001 | |
B0AT1 | 0.76 | 0.73 | 0.68 | 0.02 | 0.35 | ||
MUC2 | 0.93 | 0.94 | 0.96 | 0.02 | 0.82 | ||
PepT1 | 0.47 | 0.48 | 0.42 | 0.03 | 0.71 |
1Means within each row with different superscripts are significantly different (P < 0.05). Standard error of the mean from ANOVA (n = 6).
Thr = treatment effect; when significant, linear (L) and quadratic (Q) effects were tested.
pTOR, poultry target of rapamycin; GOT, glutamic oxaloacetic transaminase; GPT, glutamic-pyruvic transaminase; TDH, threonine dehydrogenase; ANPEP, aminopeptidase; B0AT1, neutral amino acid transporter; MUC2, mucin 2; PepT1, peptide transporter 1.