Table 1.
Oligonucleotides synthesized for amplifying the entire proviral genome of the isolate.
| Primersa | Sequences (5′-3′) | Annealing temperature (°C) | Size of PCR products |
|---|---|---|---|
| ALV-J-F | GGATGAGGTGACTAAGAAAG | 56 | 545 bp |
| ALV-J-R | CGAACCAAAGGTAACACACG | ||
| ALV-A-F | GGATGAGGTGACTAAGAAAG | 50 | 692 bp |
| ALV-A-R | AGAGAAAGAGGGGTGTCTAAGGAG | ||
| ALV-B-F | GGATGAGGTGACTAAGAAAG | 50 | 847 bp |
| ALV-B-R | ATGGACCAATTCTGACTCATT | ||
| ALV-C-F | GGATGAGGTGACTAAGAAAG | 50 | 860 bp |
| ALV-C-R | GAGGCCAGTACCTCCCACG | ||
| ALV-D-F | GGATGAGGTGACTAAGAAAG | 50 | 797 bp |
| ALV-D-R | ATCCATACGCACCACAGTATTCG | ||
| ALV-K-F | TCTGCCTCTCTACACAGTCAGCCAC | 60 | 2.0 kb |
| ALV-K-R | CTGCTTCATTCAGGTGTTCGCAATC | ||
| ALV-1F | GCCATTTGACCATTCACCAC | 50 | 2.2 kb |
| ALV-1R | ATCCCGCCTTCTGCACTGTTTAGC | ||
| ALV-2F | GGGATGGACAAACTGGGTCGGGTG | 50 | 1.7 kb |
| ALV-2R | TGGGTTGGGTGGAGAATA | ||
| ALV-3F | GTATGTAGCACCCGTAGG | 62 | 1.6 kb |
| ALV-3R | ACAAGACCAGGACACCAAT | ||
| ALV-4F | GGATGAGGTGACTAAGAAAG | 58 | 2 kb |
| ALV-4R | ACACTACATTTCCCCCTCCCTAT | ||
| ALV-5F | TGAAAGGACTGCTTTTGGGGCTTGTAGT | 58 | 0.6 kb |
| ALV-5R | TGTGGTGAATGGTCAAATGGCGTTTA | ||
| ALV-6F | TGAAAGGACTGCTTTTGGGGCTTGTAGT | 56 | 1.2 kb |
| ALV-6R | TTTTCCCGCAATAGGTTTTACAC |
a
F and R denote the forward and reverse primers for a specific fragment, respectively.