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. 2010 Jan 15;31(9):1359–1379. doi: 10.1002/hbm.20940

Figure 4.

Figure 4

Comparison of conventional Nissl stains with a combined use of multiple stains for border definition of human areas 35 and 36. The borders of area 35 with EC and area 36 are less easily appreciated in Nissl preparation (A) than in NeuN stain (B). In contrast, the borders of area 35 can be confidently placed using adjacent sections stained for NeuN (B), CB (C), and PV (D), respectively. Note the distinct CB+ columns in area 35a (C) and the extension of dense PV+ layer 3b of the EC into area 35a (D). Similarly, the border between areas 36 (36b) and TE (TEv) is less easily identified in Nissl preparation (E) than in NeuN (F) and PV (G) stains. Note in (G) the PV staining intensity and density in area TE is much greater than in area 36. For abbreviations, see list. Scale bars: 1 mm in D (A–D); 2 mm in G (E–G).