Table 3. Schedule of blood samples and volumes (in ml) for Screening to day before PfSPZ Challenge.
| Variable | Screening | Repeat PCR check
(where applicable) |
C-1 |
|---|---|---|---|
| PCR $ | 4 | 4 | 4 |
| FBC | 1 | 1 | |
| Biochemistry * | 2 | 2 | |
| Serology (HIV and
Hepatitis B) ^ |
1 | ||
| Whole blood in RNA
stabilizing buffer # |
2 | 2 | |
| Plasma/PBMCs + | 10 | 50 | |
| Volume (ml) | 20 | 4 | 59 |
| Cumulative Total (ml) | 20 | 24 | 83 |
C-1= day before PfSPZ challenge; $Includes screening for sickle cell trait, alpha-thalassaemia and other red blood cell polymorphisms and for gametocyte detection; *Biochemistry will include sodium, potassium, urea, creatinine, albumin, ALT and bilirubin; ^for assessing antibody responses; #for transcriptome analysis; +for immune responses.