Table 4. Schedule of blood samples from challenge to challenge +35 days.
| Days after
challenge |
5 | 7 | 7.5 | 8 | 8.5 | 9 | 9.5 | 10 | 10.5 | 11 | 11.5 | 12 | 12.5 | 13 | 13.5 | 14 | 14.5 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | Diag & | +24hr | +48hr | +72hr | C+35 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PCR | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | ||
| FBC | 1 | 1 | 1 | 1 | 1 | 1 | |||||||||||||||||||||||
| Biochemistry * ^ | 2 | 2 | 2 | ||||||||||||||||||||||||||
| Plasma/PBMCs $ | 30 | 30 | 30 | 30 | 30 | 30 | 50 | ||||||||||||||||||||||
| RNA analysis | 2 | 2 | 2 | 2 | 2 | 2 | |||||||||||||||||||||||
| Parasite Typing | 2 | 2 | |||||||||||||||||||||||||||
| Vol (ml) | 32 | 37 | 4 | 4 | 4 | 38 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 37 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 41 | 41 | 4 | 4 | 5 | 51 |
| Cumulative
#
Total (ml) |
115 | 152 | 156 | 160 | 164 | 202 | 206 | 210 | 214 | 218 | 222 | 226 | 230 | 234 | 238 | 275 | 279 | 283 | 287 | 291 | 295 | 299 | 303 | 344 | - | 348 | 352 | 357 | 412 |
Only one blood samples will be drawn between day of PfSPZ Challenge and C+6 (C+5). *Biochemistry will include Sodium, Potassium, Urea, Creatinine, Albumin, ALT & Bilirubin. ^Blood will be drawn for biochemistry on +9, C+21 and day of diagnosis. $Blood will be taken for plasma and/or PBMCs on +5, +7, +9, +14, +21, and day of diagnosis and then not again until C+35. &Blood sample will not be taken at diagnosis if a sample has already been taken on the same day. #The cumulative total includes pre-challenge blood volumes indicated in Table 3 (i.e. 83ml + 32 ml to give first cumulative total of 115 ml).