Table 7. List of exploratory immunological assays to be performed on the samples generated from the study a .
| Assay | Sample type | Reference |
|---|---|---|
| Antibody Dependent Respiratory Burst Assay (ADRB) | Plasma | 43, 45, 47 |
| Complement Fixation Assay | Plasma | 51 |
| DNA microarrays | Parasitised red
blood cells (RBCs) |
52, 53 |
| Growth Inhibition Assay | Plasma | 42, 43, 45 |
| Mass Cytometry (CyTOF) 1 | PBMCs | 53, 54 |
| Merozoite Opsonic Phagocytosis Assay (OPA) | Plasma | 46 |
| Multiphoton Microscopy 2 | PBMCs | 55, 56 |
| Metabolomics | Plasma | 57 |
| Protein microarrays | Plasma | 41 |
| RNA Sequencing 3 | PBMCs and
parasitised RBCs |
58 |
| Sporozoite Inhibition Assay | Plasma | 59 |
aOther assays will be conducted as and when they are readily available. 1Analysis of follicular helper T cells; 2Imaging studies of immune-modulatory effects on dendritic cell and T cell interactions; and 3T and B cell receptor repertoires including parasite transcriptomics.