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. 2019 Nov 14;10:2618. doi: 10.3389/fimmu.2019.02618

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Copyright © 2019 Schröder, Sogkas, Fliegauf, Dörk, Liu, Hanitsch, Steiner, Scheibenbogen, Jacobs, Grimbacher, Schmidt and Atschekzei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression assay of EGFP-fused NF-κB1 proteins. (A) HEK293T cells were transiently transfected with EGFP-fused mutant proteins or EGFP-fused wildtype proteins. Scale bars represent 10 μm. Immunoblots with in either nuclear or cytoplasmic fraction of transiently transfected HEK293T cells with N-terminally EGFP-tagged p50-WT or p105-WT (B) or mutant-constructs (C; as indicated) were analyzed for p105 and p50 amounts. ß-actin was used as a cytoplasmic loading control and lamin-B1 for nuclear loading control.