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. 2019 Nov 13;6:172. doi: 10.3389/fnut.2019.00172

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Copyright © 2019 Caldow, Ham, Trieu, Chung, Lynch and Koopman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Glycine attenuates muscle wasting and preserves rates of protein synthesis during nutrient starvation. Myotube diameter for cells incubated in differentiation media (DM) or HEPES buffered saline (HBS) for 5 h with increasing concentrations of glycine or isomolar concentrations of L-alanine, and representative images at the optimal dose of 2.5 mM (A). Glycine supplementation improves protein synthesis as assessed using puromycin after incubation in HBS for up to 4 h (B). Scale bar represents 50 μm. This applies to all images. Values are means ± SD, n = 4–6 per group. Significant differences are displayed where appropriate.