Skip to main content
. Author manuscript; available in PMC: 2019 Dec 10.
Published in final edited form as: Nat Microbiol. 2019 Jun 10;4(10):1737–1749. doi: 10.1038/s41564-019-0470-1

Figure 5: IFN-I signaling in IEC is required for protection from intestinal injury.

Figure 5:

Survival of Ifnar1f/f (a) and Ifnar1ΔIEC (b) mice following DSS. Mice were treated with ABX for at least 10 days prior to CR6 infection and given DSS at 10 dpi for 6 days. Survival curves were analyzed using the log-rank Mantel–Cox test. Small intestinal villus width (c) and virus levels in the SI, colon and stool (d) at 10 dpi with CR6. The following number of mice were analyzed for villus width: Ifnar1f/f ABX, n=9; Ifnar1f/f ABX+CR6, n=9; Ifnar1ΔIEC ABX, n=7 and Ifnar1ΔIEC ABX+CR6, n=9. The following number of mice were analyzed for stool titer: Ifnar1f/f, n=71 and Ifnar1ΔIEC, n=49; SI and colon titer: Ifnar1f/f, n=9 and Ifnar1ΔIEC, n=10. (e) Representative images of pSTAT3 staining in the colon and pSTAT3 expression score at day 6 post DSS. Scale bars represent 100μM. The following number of mice were analyzed for pSTAT3: Ifnar1f/f ABX, n=8; Ifnar1f/f ABX+CR6, n=6; Ifnar1ΔIEC ABX, n=6 and Ifnar1ΔIEC ABX+CR6, n=6. Figures (c) and (e) were analyzed using ANOVA with Tukey’s multiple comparisons test and figure (d) was analyzed using two-tailed t-test. All bars represent mean and error bars represent standard error of the mean. All p-values are shown in the figure.