Figure 7: MNV protects young mice from enteric bacterial infection.

Survival and weights of B6 (a) and Il22^−/− (b) mice infected with C. rodentium at 21 days old. Survival curves were analyzed using the log-rank Mantel–Cox test. For weight curves, AUC was determined for each mouse and difference between uninfected and CR6-infected groups determined by two-tailed t-test. Mean and standard error of the mean is shown for weight analysis. Bacterial titers in the stool (c), spleen (d) and liver (e) at 6 dpi with C. rodentium. The following number of mice were analyzed in (c): WT Uninfected, n=56; WT CR6, n=59; Il22^−/− Uninfected, n=48 and Il22^−/− CR6, n=47. The following number of mice were analyzed in (d): WT Uninfected, n=25; WT CR6, n=29; Il22^−/− Uninfected, n=24 and Il22^−/− CR6, n=22. The following number of mice were analyzed in (e): WT Uninfected, n=19; WT CR6, n=21; Il22^−/− Uninfected, n=23 and Il22^−/− CR6, n=23. (f) Proportion of colonic CD4⁺ and CD8⁺ T cells. (g) Proportion of colonic CD4⁺ and CD8⁺ T cells expressing IFNγ. (h) Proportion of IFNγ⁺, IL-13⁺ and IL-22⁺ cells in the colonic ILC population. The following number of mice were analyzed for cell proportions: WT Uninfected, n=12; WT CR6, n=16; Il22^−/− Uninfected, n=11 and Il22^−/− CR6, n=10. Log₁₀ transformed bacterial titers and cell proportions were analyzed using two-tailed t-tests. All bars represent mean and error bars represent standard error of the mean. All p-values are shown in the figure.