KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat anti-ChAT | Abcam | RRID:AB_2079595 |
| Mouse anti-PKCɑ | Sigma | RRID:AB_477375 |
| Mouse anti-DDK | Origene Technologies | RRID:AB_2622345 |
| Rabbit anti-GFP | Invitrogen | RRID:AB_221569 |
| Mouse anti-GPR179 | Millipore | RRID:AB_2069582 |
| Donkey anti-rabbit IgG Alexa 488 | ThermoFisher | RRID:AB_2535792 |
| Donkey anti-rabbit IgG Alexa 568 | ThermoFisher | RRID:AB_253401 |
| Donkey anti-goat IgG Alexa 488 | ThermoFisher | RRID:AB_2534104 |
| Donkey anti-goat IgG Alexa 568 | ThermoFisher | RRID:AB_2534104 |
| Bacterial and Virus Strains | ||
| AAV-EF1a-BbTagBY (AAV-Brainbow) | Addgene | Cat# 45185-AAV9 |
| AAV-EF1a-BbChT (AAV-Brainbow) | Addgene | Cat# 45186-AAV9 |
| AAV-Grm6-YFP | Johnson et al., 2017 | N/A |
| AAV-CAG-YFP | Soto et al., 2018 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) | ThermoFisher | RRID:AB_2629482 |
| DIG-RNA labeling kit | Roche | Cat# 11277073910 |
| T7 RNA polymerase | Promega | Cat# P2075 |
| RNasin | Promega | Cat# N2111 |
| SfaNI | NEB | Cat# R0172S |
| Xtremegene HP | Roche | Cat# 6366244001 |
| T7E1 | NEB | Cat# E3321 |
| T7 mMessage mMachine Ultra Kit | Life Technologies | Cat# AM1345 |
| Megaclear Kit | Life Technologies | Cat# AM1908 |
| Microcarrier gold 1.6 mm | BioRad | Cat# 1652264 |
| Tissue-Tek OCT compound | Sakura | Cat# M71484 |
| TSA Cyanine 3 (Cy3) | Perkin Elmer | Cat# SAT704A001EA |
| NBT/BCIP | Roche | Cat# 11681451001 |
| Experimental Models: Organisms/Strains | ||
| Wild-type (C57Bl6/J) | Jackson Laboratory | RRID:IMSR_JAX:000664 |
| B6;Cg-Gt(ROSA)26Sortm9(CAG-tdTomato) Hze/J (Ai9) | Jackson Laboratory | RRID:IMSR_JAX:007909 |
| B6;129S6-Chattm2(cre)Lowl/J | Jackson Laboratory | RRID:IMSR_JAX:006410 |
| Tg(Drd4-EGFP)W18Gsat | MMRRC | Cat# MGI:3839370 |
| Amigo2 KO | This study | N/A |
| Recombinant DNA | ||
| CMV-tdTomato | Clontech | 632534 |
| CMV-PSD95-YFP | Ann Marie Craig | |
| Mouse Amigo2 | Horizon Dharmacon | 105827 |
| Software and Algorithms | ||
| MATLAB | The Mathworks | RRID:SCR_001622 |
| Cogent Graphics Toolbox | Laboratory of Neurobiology | www.vislab.ucl.ac.uk |
| Fiji | Schindelin et al.,. 2012 | RRID:SCR_002285 |
| Amira | ThermoFisher | RRID:SCR_014305 |
| Offline Sorter | Plexon Inc | RRID:SCR_000012 |
| Monocle 3 | Trapnell Laboratory | https://cole-trapnell-lab.github.io/monocle3 |