FIGURE 10.

Representative double labeling immunofluorescence images of cytosolic creatine kinase (CK-B) with S-opsin in wild-type (WT) mouse retina and in Rd1 mouse retinas from postnatal day (P) 14 to P60. In WT retinas (A–C), CK-B colocalizes with S-opsin in cone inner segments (arrows), and in axonal terminals in the outer plexiform layer (arrows). At P14, CK-B colocalizes with S-opsin in shrunken inner segments and axonal terminals, with weak CK-B labeling of somas (D–F). From P30 to P60, the ONL comprises a single layer of surviving cones largely devoid of segments. CK-B colocalizes with S-opsin in cone somas and axonal terminals (G–L). At all time points, CK-B is also expressed by inner retinal neurons and Müller cells. All images were captured from the inferior retina, which comprises primarily S-cones. INL, inner nuclear layer; IS, inner segments; ONL, outer nuclear layer. Scale bar: 20 μm.