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. 2019 Nov 15;10:2572. doi: 10.3389/fimmu.2019.02572

Figure 3.

Figure 3

MS/MS spectra of the KRAS5−6/8−14 G12V spliced epitope candidate. MS/MS spectrum of the peptide KRAS5−6/8−14 G12V [KL][VVGAVGV] identified in the in vitro digestion of the synthetic polypeptide KRAS2−35 G12V and the MS/MS spectrum of the cognate synthetic peptide (left panels). The peptide sequence is shown with the corresponding b-, a- and y-ions identified. The G12V mutation is depicted in bold. In the spectra, assigned peaks for b-, a-, and y-ions are reported in color. Ion neutral loss of ammonia is symbolized by *. Red marked peaks are assigned in both in vitro digestion detected MS/MS spectrum and synthetic peptide MS/MS spectrum, whereas blue marked peaks are assigned only in one of the two spectra. The extracted ion chromatogram for the peptide identified in the in vitro digestion and the synthetic counterpart is plotted and indicates matching retention times for both peptides (right panels).