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. 2019 Nov 21;10(12):877. doi: 10.1038/s41419-019-2108-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — RUNX1 is upregulated in mesenchymal glioblastoma specimens and cell lines. a TBD0207B and TBD0220L represent samples that were removed from the back and left side of the tumor, respectively. Representative images of HE-stained tissues in TBD0207B and TBD0220L. Scale bar, 100 μm. b Representative tissues immunohistochemically stained for RUNX1 in normal brain tissue, TBD0207B and TBD0220L. Scale bar, 100 μm. Quantitative analysis of immunohistochemical staining for RUNX1 in a high-magnification view. n = 5 per group. c, d Western blots and qPCR were used to analyze the protein and mRNA expression levels, respectively, in TBD0220C, N33, N9, and U251 cells. GAPDH served as the negative control. (****p < 0.0001).