Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 21;10(12):877. doi: 10.1038/s41419-019-2108-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — The TGFβ signaling reduces the expression of MXI1 via the RUNX1/ SUV39H1 transcriptional complex, and regulates the expression of target genes (BCL3, MGP, and MXI1). a, b Real-time PCR showing the effect of shRUNX1, shRUNX1 plus TGFβ, LV-RUNX1, or LV-RUNX1 plus LY2109761 on MXI1 mRNA levels in U251 or N9 cells. c Western blots were performed to analyze the protein expression of MXI1 and H3K9me3 after treatment with shRUNX1/LV-RUNX1 or shSUV39H1/LV-SUV39H1 or rescue with TGFβ/LY2109761. GAPDH and H3 were used as loading controls. d Western blots were performed to analyze the protein expression of MXI1 and H3K9me3 after treatment with H3K9me3 inhibitor. GAPDH and H3 were used as loading controls. e ChIP-qPCR results showing the level of H3K9me3 on the MXI1 promoter after treatment with shRUNX1 or shRUNX1 plus TGFβ. f The level of H3K9me3 enrichment on the MXI1 promoter after LV-RUNX1 or LV-RUNX1 plus LY2109761. g, h Real-time PCR showing the effect of LY2109761 or TGFβ on BCL3, MGP, POSTN, and MXI1 mRNA levels in U251 or N9 cells. i Western blots showing the effect of LY2109761 or TGFβ on BCL3, MGP and MXI1 protein levels in U251 or N9 cells. (*p < 0.05, ***p < 0.001 and ****p < 0.0001, respectively).