Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 21;10(12):877. doi: 10.1038/s41419-019-2108-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — RUNX1 promotes cell proliferation, invasion and adhesion in a TGFβ pathway-dependent manner in vitro. a Wound-healing assays were used to analyze migration and invasion following treatment with shRUNX1, TGFβ plus shRUNX1, LV-RUNX1, or LY219761 plus LV-RUNX1. Scale bar, 200 μm. n = 3 per group. b N9 cells were treated with shRUNX1 or TGFβ plus shRUNX1, and U251 cells were treated with LV-RUNX1 or LY219761 plus LV-RUNX1, and Transwell assays were performed. Scale bar, 60 μm. n = 3 per group. c, d CCK8 assays and cell adhesion assays were used to analyze the proliferation and adhesion, respectively, of GBM cells. e F-actin levels were analyzed by immunofluorescence in control and shRUNX1-treated N9 cells that were transfected with EGFP lentivirus. Scale bar, 20 μm. (*p < 0.05, ***p < 0.001, and ****p < 0.0001, respectively).